The different uses and associations of don’t tread on me has made the phrase a loaded expression in contemporary political discourse.

"Chris Pratt" “Don’t Tread On Me” reflects the basic principle of individual freedom; that people want to be left alone to live their own lives without outside interference. This is somehow very controversial in 2019 where you need to conform to the mob.

— ranger (@ranger1914) July 17, 2019

Many Americans—civilians, military personnel, liberals, and conservatives—may use don’t tread on me to express national pride or champion individual rights and freedom. They may also fly the Gadsden flag featuring the motto. The phrase may appear in a variety of other imagery or products, from tattoos to bumper stickers.

I won't tread on you. Please don't tread on me. #YouDoYou #Individualism

Live Your OWN Life, No One Else's: https://t.co/8V4hMgxHZu pic.twitter.com/a8s48g59y9

— The Atlas Society (@TheAtlasSociety) August 29, 2019

Happy Day!
That’s me holding a tool I use to castrate, in front of heavy equipment I drive, wearing a shirt of the female reproductive organ that says ‘don’t tread on me’. #WomensEqualityDay #AgHag #thisiswomenswork #rancHer pic.twitter.com/3RSJMVv5SZ

— Megan Brown (@MegRaeB) August 27, 2019

The phrase don’t tread on me is associated with a variety of official political groups, including the Libertarian Party and Tea Party. Members of these groups may use don’t tread on me (and the #donttreadonme on social media) to express their beliefs, especially about small government and taxation.

It’s also associated with gun-rights activists and supporters of a broad interpretation to the Second Amendment. They may use don’t tread on me in their opposition to gun control, which they perceive to be infringing on their constitutional rights.

In the 2010s, don’t tread on me also became associated with the alt-right, who espouse white nationalism. They are seen to use don’t tread on me to promote a bigoted vision of race and power in America.

We don't talk enough about the Tea Party as the major precursor/progenitor of the alt-right. I still see "Don't Tread on Me" plates everywhere, and we just don't talk about how that language and symbolism has directly contributed to the rehabilitation of fascism in America. https://t.co/1Ze959FBvd

— Peter Z Grimm (@The_Slavsquatch) August 28, 2019

Don’t tread on me is referenced elsewhere in culture, too. Metallica released a track in 1991 called “Don’t Tread on Me,” which prominently featured the phrase (and alluded to the Gadsden flag) in its lyrics:

Liberty or Death
What we so proudly hail
Once you provoke her
Rattling of her tail

Never begins it
Never, but once engaged

Never surrenders
Showing the fangs of rage

I sad, “Don’t tread on me”

In a 1995 episode of The Simpsons, Bart writes don’t tread on me on his rear end, which he flashes at angry Australians after he escapes punishment from their government.

In the 2010s, the Gadsden flag inspired many parody memes. One substituted a red Lego for the snake. (Because stepping on Lego, as many of us know so well, hurts!) Another, depicting a giant foot stepping on the rattlesnake, riffed on the motto: “I specifically requested the opposite of this.” The snek meme has also inspired some interpretations, such as “no step on snek.”