will.i.am has accused a Qantas flight attendant of racism while on board a flight from Brisbane to Sydney on Saturday.

The 44-year-old performer - real name William Adams - shared a scathing Twitter rant to fans after he believed he was the victim of racially-motivated abuse.

Will.i.am, who is visiting Australia as part of the RNB Fridays Live festival with his band Black Eyed Peas, also complained about having police greet him at the terminal after being 'polite' and respectful to crew members during his ordeal.

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The star wrote on Twitter: 'I'm sorry to say me and my group have experienced the worst service due to an overly aggressive flight attendant.'

'I don't want to believe she racist. But she has clearly aimed all her frustrations only at the people of colour.'

He then went on to specifically name the flight attendant and shared a shaky photo of a federal police officer, who greeted the group on disembarking the plane.

'This is how you're greeted when you land from Brisbane to Sydney flying @qantas with a #RacistFlightattendant,' he wrote.

'She sent the police after me because I couldn't hear the P.A while making beats on the plane wearing noise cancelling headphones.'

However, a witness to the incident - who does not wish to be named - told the Daily Telegraph the stoush escalated when 50 Cent's guitarist Travis Ferguson was told he would have to check his instrument in rather than take it on board.

The passenger said Ferguson left the plane and returned with his guitar, leading to a verbal altercation that will.i.am was oblivious to because he could not hear it through his noise-cancelling headphones.

According to the passenger, the incident between the Black Eyed Peas star and the flight attendant he named occurred shortly afterwards. 

However, many were quick to jump to the defence of the flight attendant and criticised the star for naming and shaming her to his millions of followers. 

Australian entertainment reporter Peter Ford was one of those most vocal in his criticism - calling on the rapper to tell his followers to stop abusing the Qantas employee he named.

'Some of your many millions of followers have already starting abusing this woman. Hoping you ask them to stop,' Mr Ford said. 

Following a brief Twitter exchange with the reporter, where the Where Is The Love singer defended his decision to publicly name the flight attendant, will.i.am posted an appeal to his followers to end the verbal tirade.

'This type of disrespect and name calling is uncalled for - I don't support abuse and attacks like this,' he wrote at about 5pm. 

'I hope that everyone can be more compassionate & understanding towards one another...because it was the lack of compassion that caused this.'

'I'm sorry? Is callin' the police on a passenger for not hearing he P.A due to wearing noise cancelling headphones appropriate?' he wrote.

'If I didn't put away my laptop 'in a rapid 2min time' I'd understand. I did comply quickly & politely, only to be greeted by police. I think I was targeted.' 

Qantas hit back in a statement and laid the blame on the performer.

'There was a misunderstanding on board, which seems to have been exacerbated by will.i.am wearing noise cancelling headphones and not being able to hear instructions from crew,' it wrote.

'We completely reject the suggestion this had anything to do with race.

'We'll be following up with will.i.am and wish him well for the rest of the tour.' 

The Australian Federal police confirmed to Daily Mail Australia on Saturday that they had responded to a request for assistance from an airline arriving into Sydney from Brisbane and spoke to the crew and passengers on board.

'AFP officers met the flight on arrival and spoke to crew and passengers on board. No further action was required as a result. The AFP considers this matter finalised,' a spokesperson said in a statement.

The woman believed to be the flight attendant has been contacted for comment.